Total RNA Isolation Kit (Tissue)
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Total RNA Isolation Kit (Tissue)
Total RNA Isolation Kit (Tissue)
For research use only
Sample: up to 30 mg of tissue, up to 25 mg of paraffin-embedded tissue
Yield : up to 30μg
Introduction
The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from tissue sample. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the the glass fiber matrix of the spin column (1) while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with REL Buffer without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection. The entire procedure can be completed within 25-40 minutes.
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Total RNA Isolation Kit (Plant)
Total RNA Isolation Kit (Plant) For research use only Sample: 100 mg of fresh plant tissue or 25 mg of dry plant tissue Yield: up to 30μg IntroductionThe Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from plant samples. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species longer than 100 bases to bind to the the glass fiber matrix of the spin column . The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection . The entire procedure can be completed within 60 minutes.
Total RNA Isolation Kit (Blood/Cell/Bacteria)
Total RNA Isolation Kit (Blood/Cell/Bacteria) For research use only Sample: up to 300 μl of whole blood, 107mammalian cells and 109 bacterial cells Yield: up to 30μg Introduction The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from whole blood, mammalian cells and bacterial cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the the glass fiber matrix of the spin column (1) while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with REL Buffer without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection .The entire procedure can be completed within 25-40 minutes.
