Taq DNA Polymerase
產品介紹
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Taq DNA Polymerase
Taq DNA Polymerase
詳細介紹:
Cat No. TQ0500
Size500 units Conc.5 units/μlStore at -20°C
Description
Taq DNA Polymerase is purified from E. coli. expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single- stranded templates in the presence of a primer.
Contents
Taq DNA Polymerase 100 ul(5 units/ul)
10X PCR buffer 1.5ml
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PCR All in One
Cat. No. PQ0100 Size: 100 reactions (4 X 1.125ml)Store at -20°CDescription PCR All in One contains Mg++, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient to allow amplification during PCR.PCR All in One may be stored at either -20°C or 4°C. Storage at 4°C avoids the necessity of thawing the mix before assembling the PCR. No detectable reduction of PCR performance or enzyme activity is observed after storage of PCR All in One for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance. For research use only
HotStar All in One
Cat. No. HQ0100 Size: 100 reactionsStore at 4 or -20°CDescription HotStar All in One provides qualified reagents for the amplification of nucleic acid templates by polymerase chain reaction (PCR). The mixture contains anti-Taq DNA polymerase antibody, Mg++, dNTPs, and recombinant Taq DNA polymerase at concentrations sufficient to allow amplification during PCR. HotStar All in One is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50 μl each are provided. Due to specific binding of the antibody, HotStar All in One is present in an inactive form and is reactivated after a denaturation step in PCR cycling at 94°C. HotStar All in One may be stored at either -20°C or 4°C. No detectable reduction of PCR performance or enzyme activity is observed after storage of HotStar All in One for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance or activity.
HiFi DNA Polymerase
Cat. No. HI0100 Size: 100 Reactions Conc. 1 U/μl Store at -20°CDescription HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kb). HiFi has higher stability at high temperature. Users may program the initial denaturizing at even 100°C for 2 min, and this property makes HiFi perform very well for GC-rich PCR. NoteHiFi DNA Polymerase produces blunt end PCR products. Add conventional Taq polymerase at the final step for further TA or GC cloning application.
