Protein Ladder(10-245kDa)
產品介紹
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Protein Ladder(10-245kDa)
Cat. No. PL0501
Size: 500 μl
Store at -20°C
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well mini-gel
respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (> 1.5 mm) or larger gel.
Description
The10-245kDa Prestained Protein Ladderis a three-color protein standard with 12 pre stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The 10-245kDa Prestained Protein Ladderis designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25°C), 2 % SDS, 3.6 M Urea,1 mM 2-Mercaptoethanol, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, 10-245kDa Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
Note
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each
electrophoresis condition
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Protein Ladder(3.5-245kDa)
Cat. No. PL0502 Size: 500 μl Store at -20°C • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well mini-gel respectively. • 1.5~2.5 μl per well for general Western transferring. • Apply more for thicker (> 1.5 mm) or larger gel.Description The 3.5-245kDa Prestained Protein Ladderis a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).The 3.5-245 kDa Prestained Protein Ladderis designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).Quality Control Under suggested conditions, 3.5-245kDa Prestained Protein Ladderresolves 13 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
Protein Ladder(10-180kDa)
Cat. No. PL0500 Size: 500 μlStore at -20°C for 24 months • 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15 well or 10 well mini-gel respectively. • 2~3 μl per well for general Western transfering. • Apply more for thicker (>1.5 mm) or larger gel. DescriptionThe 10-180kDa Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The 10-180kDa Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes(PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. ContentsApproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) glycerol). Quality Control 5 μl of 10-180kDa Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.NoteThe apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in eachelectrophoresis condition
